[~{U*R*~}]~{!!~}

~{D?5D#:L=LVRH5:KXQyIz3$RrWS"qJ\Le&AA40POrWh6O6T7NOY0)O80{~}SPC-A-1~{5DS0Ol!#~}

~{7=7(#:S&SCGW:M2cNvVy:MTS=;Av<IGF~{"q~}R~{&AA45%?KB!?9Le#(~}IGF~{"q~}R-~{&A~}Mab~{#)!#=+J5QiO80{Vj~}SPC-A-1~{O80{7VN*J5QiWi:M?U0W6TUUWi#,J5QiWi8xSh~}20~{!"~}40~{!"~}60~{!"~}80~{!"~}100~{!"~}120~{!"~}140~{!"~}160~{!"~}180~{!"~}200 ng/ml~{2;M,E(6H5D~}IGF~{"q~}R-~{&A~}Mab~{8IT$!#7V1pSZ5Z~}0~{!"~}24~{!"~}48~{!"~}72~{P!J1H!3vR;?iE`Qx0e=xPPSPC-A-1 MTT~{GzO_!"O80{PNL,Q'!"3,N"=a99!"O80{V\FZ5DS0Ol!#~}

~{=a9{#:~}IGF~{"q~}R-~{&A~}Mab~{8IT$:sO80{5rMvCwOTTv%~{#)7V1pN*~}10.52~{!@~}2.66 vs 5.73~{!@~}1.88~{#(~}P=0.009~{#)!#O80{TvV3BJJ\5=RVVF!#~}0~{!+~}160 ng/ml~{E(6H76N'DZ#,KfWE?9LeE(6H5D=55M#,FdRVVFBJO`S&OB=5#,4fTZCwOT5D200 ng/ml~{J1#,Tv4sE(6HFdRVVFBJN4<{CwOTLa8_#(T<~}80%~~85%~{76N'#)!#~}

~{=aB[#:1>QP>?LaH!5D~}IGF1-~{&A~}R~{5%?KB!?9Le6T~}IGF1R~{&AA4>_SP=O:CGW:MA&!#M(9}0POrWh6O~}IGF1R~{VP5D&AA49&D\6TRVVF~}SPC-A-1~{7NOY0)O80{VjTvV3!"4Y=x5rMvTZLeMbJG?IPP5D#;LaJ>Uk6T~}IGF1-~{&A~}R~{5%?KB!?9Le0POrVNAFJG7GP!O80{7N0)IzNoVNAFJGR;8vV55C9XW"5DQP>?7=Or!#~}

[~{9X<|4J~}]~{!!RH5:KXQyIz3$RrWS"qJ\Le#;5%?KB!?9Le#;O80{Vj#;0POrVNAF#;O80{5rMv~}

[Abstract]~{!!~}

Purpose~{#:~}To study the effect of insulin-like growth factor 1 receptor ~{&A~}-strain(IGF1R-~{&A~}) interrupted by a special antibody ~{#(~}IGF~{"q~}R-~{&A~}Mab~{#)~}on lung adenoma cell line SPC-A-1.

Methods~{#:~}IGF~{"q~}R-~{&A~}Mab was extracted by hybrid technology. SPC-A-1 cells were separated into 2 groups,the IGF~{"q~}R-~{&A~}Mab and the blank control. The IGF~{"q~}R-~{&A~}Mab cells were interfered by different densities of IGF~{"q~}R-~{&A~}Mab,including 20,40,60,80,100,120,140,160,180 and 200 ng/ml. The MTT curve line, morphology, ultrastructure and cell cycles were observed at 0,24,48,72 hours after the intervention respectively.

Results~{#:~}Compared with the control, apoptosis in IGF~{"q~}R-~{&A~}Mab group was significant~{#(~}P=0.009~{#)~}and proliferation rate was decreased obviously within 160 ng/ml. However, the proliferation rate was no significant when the special antibody density was more than 200 ng/ml.

Conclusions~{#:~}The affinity of IGF~{"q~}R-~{&A~}Mab at IGF1R ~{&A~}-strain is high.The interruption of IGF1R ~{&A~}-strain by IGF~{"q~}R-~{&A~}Mab shows the obvious biological effects in vitro,with inclusion of promoting apoptosis and suppressing proliferation, which indicate the interruption targeting IGF1R ~{&A~}-strain is prospective for non-small-cell lung carcinoma.

[Key words]~{!!~}insulin-like growth factor 1 receptor;monoconal antibody;cell lines; targeting treatment;apoptosis



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