| 胃癌组织中EB病毒的检测及其增殖期基因的表达 |
| 作者：王云，罗… 文章来源：《中国癌症杂志》 点击数： 更新时间：2005-5-17 |
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摘要] 目的：探讨EB病毒（Epstein-Barr virus, EBV）感染与胃癌发生的关系及其增殖期基因在EBV阳性胃癌发生中的作用。
方法：应用聚合酶链反应(PCR)-Southern杂交检测185例胃癌组织和相应癌旁组织中特异性EBV DNA片段，PCR阳性标本用原位杂交(ISH)技术检测石蜡切片组织中EBV编码小RNA 1（EBER1）的表达，以确定EBV阳性胃癌。再应用RTPCR和Southern杂交技术检测EBV增殖期基因（即刻早期基因BZLF1、BRLF1，早期基因BARF1、BHRF1，晚期基因BcLF1、BLLF1）的表达。
结果：13例胃癌组织EBV阳性（7.03%），癌旁组织未检测到EBV感染，胃癌和癌旁组织中EBV阳性率有显著性差异（χ2=11.0769， P=0.0009）。EBV阳性和阴性胃癌在年龄、病理类型、临床分期、淋巴结转移和发生部位的差别均无显著性(P=0.973, 0.141, 0.259, 0.586, 0.062)，但性别之间的差异有显著性，男性EBV阳性率高于女性（χ2=5.2317，P=0.021）。增殖期基因中即刻早期基因BZLF1有6例表达阳性，而BRLF1均为阴性；早期基因中有6例BARF1表达阳性，2例BHRF1表达阳性；晚期基因BcLF1有7例表达阳性，而BLLF1均为阴性。
结论：EBV感染与胃癌的发生有一定的相关性，部分EBV阳性胃癌组织中存在EBV增殖性感染，早期基因BARF1和BHRF1在EBV阳性胃癌发生过程中可能有重要作用。
[关键词] EB病毒；胃癌；增殖期基因；RT-PCR；原位杂交
[Abstract] Purpose：To explore the relationship between Epstein-Barr virus(EBV) infection and gastric carcinoma and the role of EBV lytic genes in the tumorigenesis of EBV-gastric carcinomas.
Methods：185 gastric carcinoma tissues and 185 corresponding para-carcinoma tissues were tested for EBV genome by polymerase chain reaction (PCR)-southern analysis. EBV-encoded small RNA 1(EBER1) of the PCR positive specimens was detected by in situ hybridization (ISH). Gastric carcinoma with positive EBER1 signals was confirmed as EBV-positive gastric carcinoma. RT-PCR and Southern blotting were used to detect the expression of EBV lytic genes (immediately early genes BZLF1 and BRLF1, early genes BARF1 and BHRF1, late genes BcLF1 and BLLF1) in EBV-positive gastric carcinomas.
Results：There were 13 EBV positive samples in gastric carcinomas (7.03%). No EBV positive sample was found from corresponding para-carcinomas. The difference of the EBV positivity was significant between carcinoma and corresponding adjacent carcinoma tissues(χ2=11.0769，P=0.0009). In our series, age, pathological differentiation, clinical stages, lymph node metastasis and location of cancer were not different between EBV-positive and EBV-negative gastric carcinomas in (P=0.973, 0.141, 0.259, 0.586, 0.062, respectively), while sex was significantly different between EBV-positive and EBV-negative gastric carcinomas(χ2=5.2317，P=0.021). The EBV positivity of male was higher than that of female. Of the 13 EBV-associated samples, 7 exhibited BcLF1 transcript and 2 exhibited BHRF1 transcript. The transcripts of BZLF1 were detected in 6 cases, and those of BARF1 also in 6 cases. No BLLF1 and BRLF mRNA were detected in the 13 EBV-positive samples.
Conclusions：EBV infection is associated with the development of gastric carcinoma. Lytic EBV infection occurs in part of the EBVassociated gastric carcinomas, and early genes BARF1 and BHRF1 may play an important role in the tumorigenesis of the EBV-positive gastric carcinoma.
[Key words] Epstein-Barr virus；gastric carcinoma；lytic gene；PT-PCR；ISH
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